Chemical VS Biological Indicators – Why do I need both?
At Woodhouse Laboratories, we understand that proper sterilization is non-negotiable for safety and compliance. That’s why we proudly supply both chemical and biological indicators, offering a comprehensive, two-tiered approach to sterilization monitoring. We provide you with the tools for both quick, immediate feedback and the definitive, “gold standard” proof of success.
Understanding Our Indicators
| Indicator Type | Definition & Purpose | Key Function |
| Chemical Indicators | A chemical indicator shows that a sterilization process has been exposed to certain conditions, such as temperature or pressure. | To provide a quick, visual, and immediate confirmation that a sterilization cycle has run. They do not, however, guarantee sterility. |
| Biological Indicators | A biological indicator uses highly resistant microorganisms to prove that sterilization was successful. | To offer the highest level of assurance by verifying the actual killing of the most resistant microorganisms—the ultimate confirmation that the process worked. |
Woodhouse Chemical Indicators: The Quick Check

Our chemical indicators are designed for rapid, routine monitoring.
| Feature | Description |
| Function | To provide an immediate, visual cue that a sterilization cycle has reached specific parameters (e.g., temperature, pressure, or chemical exposure). |
| Mechanism | They contain a chemical that visibly changes color or appearance when exposed to the required conditions. |
| Examples | Indicator tape, strips, or cards for placement inside or outside packages. |
| Limitations | They only confirm that the process parameters were met; they do not confirm the actual destruction of all microorganisms. |
| Use Case | Ideal for routine monitoring and quick checks of individual packages to confirm the cycle started and the conditions were reached. |
Woodhouse Biological Indicators: The Gold Standard

Our biological indicators are the “gold standard” for sterilization monitoring, providing the definitive proof of efficacy.
| Feature | Description |
| Function | To verify that a sterilization process is effective and capable of killing highly resistant microorganisms. |
| Mechanism | They contain a specific, highly resistant microorganism (like bacterial spores of Geobacillus stearothermophilus or Bacillus atrophaeus). After the cycle, the indicator is incubated. A negative result (no growth) confirms successful sterilization. |
| Examples | Spore strips or self-contained spore vials. |
| Limitations | The results are provided after a time-consuming incubation period. |
| Use Case | Essential for validating sterilization processes and for routine monitoring to ensure your sterilizer is working properly and consistently. |
The Woodhouse Key Takeaway: Use Both!
It is standard, best practice to use both chemical and biological indicators together. This strategy ensures you receive both immediate feedback and definitive confirmation of sterilization.
- Chemical Indicators answer the question: “Did the process run?”
- Biological Indicators answer the question: “Did it work?”
Trust Woodhouse Laboratories to provide the complete solution for your sterilization monitoring needs!