Dental Unit Waterline Testing

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An easy and reliable method to evaluate your dental irrigant water quality.


  • Easy to use: Collect water samples in the collection vials and return in the provided insulated return pouch


  • Reliable: Provides third-party documentation of dental unit waterline water quality based on heterotrophic plate counts


  • Fast Results: Comprehensive laboratory report emailed after 72 hours of sample receipt

It's simple to signup and test with BiofilmAlert.

Here's how to do it in three easy steps:

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Place an order online

Order as many, or as few, testing vials as you need depending on the size of your clinic. You'll need 1 vial per device and/or water source.

Collect your water samples

Take a water sample directly from each device and/or water source and ship your testing vials to our lab using the included prepaid UPS overnight shipping label.


View your results online

Results are reported by email approximately 72 hours after sample is received.

What is biofilm?

Biofilms are microorganisms that can grow on many different surfaces. Microorganisms that form biofilms include bacteria and fungi. Biofilms thrive in wet environments.

Over the course of regular use, waterlines and connected devices such AW syringes can build up biofilms which contaminate the water which flows through them.  The result of this can be healthcare acquired infection.

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Our Methods

We use Multiple Enzyme Technology (MET) to detect heterotrophic plate count (HPC) bacteria in water. The most common enzymes of waterborne bacteria are all targeted for detection.

Standard method 9215E is U.S. EPA approved and included in Standard Methods for Examination of Water and Wastewater.

Correlates with U.S. EPA-approved, pour-plate method using plate count agar and 48-hour, 35°C incubation. Internal comparisons to Standard Methods (9215B) pour-plate method show a correlation coefficient (r) of 0.97.

CDC Recommendations for Dental Unit Water Quality

  1. Use water that meets EPA regulatory standards for drinking water (i.e., ≤ 500 CFU/mL of heterotrophic water bacteria) for routine dental treatment output water.
  2. Consult with the dental unit manufacturer for appropriate methods and equipment to maintain the quality of dental water.
  3. Follow recommendations for monitoring water quality provided by the manufacturer of the unit or waterline treatment product.
  4. Use sterile saline or sterile water as a coolant/irrigant when performing surgical procedures.

BiofilmAlert Dental Unit Waterline Testing

Base Kit (1 sample vial)

What’s Included:

  • 1 Sterile Sample Vial
  • Insulated Mailing Pouch
  • 2 Ice Packs
  • Chain of Custody
  • Illustrated Instructions
  • Laboratory Testing
  • Overnight Return Postage to Laboratory
Each additional vial

Select 1 additional vial per device and/or source


Sign up for our subscription service and receive a new test kit automatically every 3 months.

Save 5% – and never forget about your quarterly compliance testing again!

BiofilmAlert - an easy and reliable method to evaluate your dental irrigant water quality.

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